Journal: Microorganisms

Article Title: Structural and Functional Dynamics of Staphylococcus aureus Biofilms and Biofilm Matrix Proteins on Different Clinical Materials

doi: 10.3390/microorganisms7120584

Figure Lengend Snippet: Atomic Force Microscopy (AFM) topographical images of the materials. Borosilicate glass (G), plexiglass (PG), hydroxyapatite (HA), titanium (TI) and polystyrene (PS) captured with the image size of 50 × 50 µm ( A ). Zoomed images of the materials with the size of 20 × 20 µm ( B ).

Article Snippet: Biofilms were formed on 96-well plates (96-WPs) (NunclonTM Δ surface polystyrene plates, NuncTM, Roskilde, Denmark) or on coupons made of borosilicate glass (G), plexiglass (PG), titanium (TI) with dimensions: 0.4 cm height, 1.27 cm diameter; and hydroxyapatite (HA) with dimensions: 0.25 cm height, 1.27 cm diameter (BioSurface Technologies Corporation, Bozeman, MT, USA).

Techniques: Microscopy

Journal: Microorganisms

Article Title: Structural and Functional Dynamics of Staphylococcus aureus Biofilms and Biofilm Matrix Proteins on Different Clinical Materials

doi: 10.3390/microorganisms7120584

Figure Lengend Snippet: Selected roughness parameters of materials used in the assays. S dr describes the relative increase in surface area compared to the flat surface. V v reflects void volume, describing the openness of the surface or surface porosity.

Article Snippet: Biofilms were formed on 96-well plates (96-WPs) (NunclonTM Δ surface polystyrene plates, NuncTM, Roskilde, Denmark) or on coupons made of borosilicate glass (G), plexiglass (PG), titanium (TI) with dimensions: 0.4 cm height, 1.27 cm diameter; and hydroxyapatite (HA) with dimensions: 0.25 cm height, 1.27 cm diameter (BioSurface Technologies Corporation, Bozeman, MT, USA).

Techniques:

Journal: Microorganisms

Article Title: Structural and Functional Dynamics of Staphylococcus aureus Biofilms and Biofilm Matrix Proteins on Different Clinical Materials

doi: 10.3390/microorganisms7120584

Figure Lengend Snippet: Formation of Staphylococcus aureus ATCC 25923 on five substrate materials. Bacterial attachment is expressed as log 10 values of CFU·(mL·cm 2 ) −1 . Differences in attachment were assessed for a single material between different time points, and for a fixed time point between the different materials, using one-way ANOVA with Games–Howell post-test. *, significant difference ( p < 0.05). Error bars denote the standard error of the mean (SEM) ( n = 3). G, borosilicate glass; PG, plexiglass; HA, hydroxyapatite; TI, titanium; PS, polystyrene.

Article Snippet: Biofilms were formed on 96-well plates (96-WPs) (NunclonTM Δ surface polystyrene plates, NuncTM, Roskilde, Denmark) or on coupons made of borosilicate glass (G), plexiglass (PG), titanium (TI) with dimensions: 0.4 cm height, 1.27 cm diameter; and hydroxyapatite (HA) with dimensions: 0.25 cm height, 1.27 cm diameter (BioSurface Technologies Corporation, Bozeman, MT, USA).

Techniques:

Journal: Microorganisms

Article Title: Structural and Functional Dynamics of Staphylococcus aureus Biofilms and Biofilm Matrix Proteins on Different Clinical Materials

doi: 10.3390/microorganisms7120584

Figure Lengend Snippet: Wheat germ agglutinin Alexa Fluor ® 488 conjugate-based quantification of poly- N -acetyl-β-(1-6)-glucosamine (PNAG) in S. aureus ATCC 25923. Differences in PNAG contents (expressed as relative fluorescence units (RFUs)) were assessed for a single material between different time points, and for a fixed time point between the different materials, using one-way ANOVA comparisons and Games–Howell post-tests for blank-corrected data points. *, significant difference ( p < 0.05). Error bars denote the standard error of the mean (SEM) ( n = 2). G, borosilicate glass; PG, plexiglass; HA, hydroxyapatite; TI, titanium; PS, polystyrene.

Article Snippet: Biofilms were formed on 96-well plates (96-WPs) (NunclonTM Δ surface polystyrene plates, NuncTM, Roskilde, Denmark) or on coupons made of borosilicate glass (G), plexiglass (PG), titanium (TI) with dimensions: 0.4 cm height, 1.27 cm diameter; and hydroxyapatite (HA) with dimensions: 0.25 cm height, 1.27 cm diameter (BioSurface Technologies Corporation, Bozeman, MT, USA).

Techniques: Fluorescence

Journal: Microorganisms

Article Title: Structural and Functional Dynamics of Staphylococcus aureus Biofilms and Biofilm Matrix Proteins on Different Clinical Materials

doi: 10.3390/microorganisms7120584

Figure Lengend Snippet: Protein concentrations mg·(mL·cm 2 ) −1 ( A ) and the number of the identified matrix-associated proteins (detected in both replicates) ( B ) of the S. aureus ATCC 25923 biofilms. Differences in protein concentrations were assessed for a single material between different time points, and for a fixed time point between the different materials, using one-way ANOVA comparisons and Tukey post-tests for blank-corrected data points. *, significant difference ( p < 0.05). Error bars denote the standard error of the mean (SEM) ( n = 2). G, borosilicate glass; PG, plexiglass; HA, hydroxyapatite; TI, titanium; PS, polystyrene.

Article Snippet: Biofilms were formed on 96-well plates (96-WPs) (NunclonTM Δ surface polystyrene plates, NuncTM, Roskilde, Denmark) or on coupons made of borosilicate glass (G), plexiglass (PG), titanium (TI) with dimensions: 0.4 cm height, 1.27 cm diameter; and hydroxyapatite (HA) with dimensions: 0.25 cm height, 1.27 cm diameter (BioSurface Technologies Corporation, Bozeman, MT, USA).

Techniques:

Journal: Microorganisms

Article Title: Structural and Functional Dynamics of Staphylococcus aureus Biofilms and Biofilm Matrix Proteins on Different Clinical Materials

doi: 10.3390/microorganisms7120584

Figure Lengend Snippet: The chemotolerance of 18- and 66-h-old S. aureus ATCC 25923 biofilms formed on hydroxyapatite, HA ( A ) and titanium, TI ( B ) and exposed to several antibiotics for 2 or 24 h. The results are expressed as log 10 values of CFU·(mL·cm 2 ) −1 . Plain white and dark grey bars illustrate 18- and 66-h-old biofilms (respectively) with 2-h-long antibiotic exposure, while striped white and dark grey bars illustrate 18- and 66-h-old biofilms (respectively) with 24-h-long antibiotic exposure. The results of antibiotic-treated biofilms were compared to TSB-treated biofilms. The statistical analysis was performed by using unpaired t-tests with Welch’s correction. * p < 0.05 and *** p < 0.001 were considered statistically significant and highly significant, respectively. Error bars denote the standard error of the mean (SEM) ( n = 2).

Article Snippet: Biofilms were formed on 96-well plates (96-WPs) (NunclonTM Δ surface polystyrene plates, NuncTM, Roskilde, Denmark) or on coupons made of borosilicate glass (G), plexiglass (PG), titanium (TI) with dimensions: 0.4 cm height, 1.27 cm diameter; and hydroxyapatite (HA) with dimensions: 0.25 cm height, 1.27 cm diameter (BioSurface Technologies Corporation, Bozeman, MT, USA).

Techniques:

Journal: Microorganisms

Article Title: Structural and Functional Dynamics of Staphylococcus aureus Biofilms and Biofilm Matrix Proteins on Different Clinical Materials

doi: 10.3390/microorganisms7120584

Figure Lengend Snippet: The chemotolerance of S. aureus ATCC 25923 as quantified using the log reduction of viable counts. The 18-h- and 66-h-old biofilms were formed on hydroxyapatite (HA) and titanium (TI), and exposed to penicillin G, levofloxacin, doxycycline and vancomycin.

Article Snippet: Biofilms were formed on 96-well plates (96-WPs) (NunclonTM Δ surface polystyrene plates, NuncTM, Roskilde, Denmark) or on coupons made of borosilicate glass (G), plexiglass (PG), titanium (TI) with dimensions: 0.4 cm height, 1.27 cm diameter; and hydroxyapatite (HA) with dimensions: 0.25 cm height, 1.27 cm diameter (BioSurface Technologies Corporation, Bozeman, MT, USA).

Techniques: